Abstract
INTRODUCTION: Pyriproxyfen is an insect growth regulator that has proved promising for controlling vector mosquitoes such as Culex, Anopheles, and Aedes species. In this study, the efficacy of pyriproxyfen at different concentration were evaluated against dengue vectors, Aedes aegypti and Aedes albopictus. METHODS: Eggs, larvae and pupae were exposed to 100, 50, 40, 30, 20, 10, 5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2 and 0.1mg/L of pyriproxyfen. Bioassay was carried out to determine the concentration-mortality response of immature stages of Aedes mosquito towards pyriproxyfen. RESULTS: Following the exposure, 100% of eggs from both species were prevented from hatching in all petri dish treated containing at least 0.7mg/L of pyriproxyfen while ~95% of eggs hatched from the control group within 24 hours. Within 48 hours, larvae from both species treated with minimum 3mg/L of pyriproxyfen shows 100% mortality with LC50 recorded in Ae. aegypti and Ae. albopictus are 0.740 (0.683-0.803) and 0.901 (0.817-1.000) respectively. LC50 for pupae in Ae. aegypti and Ae. albopictus are 0.924 (0.789-1.092) and 1.183 (1.027-1.380) with minimum 4 mg/L of pyriproxyfen required for 100% mortality within 48 hours. An additional study showed that 100 gravid female mosquitos, exposed to 100mg/L pyriproxyfen transfer enough chemicals to new oviposition sites containing uncontaminated water to prevent 100% of adult emergence. The existence of pyriproxyfen in uncontaminated water is assessed using Ultra Performance Liquid Chromatography (UPLC). DISCUSSION: The actual efficacy of insecticide in field application can be reflected from laboratory test and evaluation.
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@proceedings{APCPH-2019-167,
title = {Evaluation of Different Concentration of Pyriproxyfen, An Insect Growth Regulator (IGR) Against Immature Stages of Aedes Aegypti and Aedes Albopictus},
author = {Noor Adila Mohamad and Intan Haslina Ishak and Hadura Abu Hasan},
year = {2019},
date = {2019-07-22},
urldate = {2019-07-22},
journal = {6th Asia-Pacific Conference on Public Health 2019 Proceedings},
issue = {6},
abstract = {INTRODUCTION: Pyriproxyfen is an insect growth regulator that has proved promising for controlling vector mosquitoes such as Culex, Anopheles, and Aedes species. In this study, the efficacy of pyriproxyfen at different concentration were evaluated against dengue vectors, Aedes aegypti and Aedes albopictus. METHODS: Eggs, larvae and pupae were exposed to 100, 50, 40, 30, 20, 10, 5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2 and 0.1mg/L of pyriproxyfen. Bioassay was carried out to determine the concentration-mortality response of immature stages of Aedes mosquito towards pyriproxyfen. RESULTS: Following the exposure, 100% of eggs from both species were prevented from hatching in all petri dish treated containing at least 0.7mg/L of pyriproxyfen while ~95% of eggs hatched from the control group within 24 hours. Within 48 hours, larvae from both species treated with minimum 3mg/L of pyriproxyfen shows 100% mortality with LC50 recorded in Ae. aegypti and Ae. albopictus are 0.740 (0.683-0.803) and 0.901 (0.817-1.000) respectively. LC50 for pupae in Ae. aegypti and Ae. albopictus are 0.924 (0.789-1.092) and 1.183 (1.027-1.380) with minimum 4 mg/L of pyriproxyfen required for 100% mortality within 48 hours. An additional study showed that 100 gravid female mosquitos, exposed to 100mg/L pyriproxyfen transfer enough chemicals to new oviposition sites containing uncontaminated water to prevent 100% of adult emergence. The existence of pyriproxyfen in uncontaminated water is assessed using Ultra Performance Liquid Chromatography (UPLC). DISCUSSION: The actual efficacy of insecticide in field application can be reflected from laboratory test and evaluation.},
note = {Type: POSTER PRESENTATION; Organisation:},
keywords = {Aedes aegypti, Aedes albopictus, Insect growth regulator, pyriproxyfen},
pubstate = {published},
tppubtype = {proceedings}
}