Abstract
Background: The quality checking of blood film for malaria parasite is an important activity for monitoring the performances of malaria microscopy. Considering its important roles in the Malaria Elimination Programme in Malaysia, we evaluated the performance of the quality checking of blood film malaria parasite carried out between 2015 to 2021 and analyze the outcomes. Methodology: A total of 67,138 blood films malaria parasite were received and analysed for quality checking at the National Public Health Laboratory, of which a total of 18,550 and 48,588 were positive and negative slides, respectively. The microscopy examinations were performed by WHO level 1 (expert) certified malaria microscopist. Results: Analysis showed 99.82% or 67,015 slides were in good agreement with the National Public Health Laboratory readings and only 0.19% or 126 slides were considered as false reading or errors. Of the 126 false reading or errors, 105 were from the positive slides while another 21 were from the negative slides. The percentages of false positive and false negative slides were at 0.17% and 0.04%, respectively. Conclusion: There are still inaccuracies in the diagnosis of all species of malaria parasites, especially Plasmodium knowlesi. The present assessment also revealed that there is a needs for continuous refresher training on malaria microscopy, monitoring of the quality of blood smears preparation and to strictly adhere to the standard procedures of using blood in EDTA tube for preparing the blood film for malaria parasite. In general, the performance of quality checking for blood film malaria parasite are generally satisfactory with the percentage of errors lower than 1%, below the standard set by the National Malaria Elimination Programme, Ministry of Health Malaysia.
Links
- https://apcph.cphm.my/wp-content/uploads/2022/07/APCPH2022-P-8.pdf
- https://apcph.cphm.my/wp-content/uploads/wpforms/1176-1e04940bb5d885bf8711ed1909[...]
BibTeX (Download)
@proceedings{APCPH2022-P-8,
title = {A Seven Year Analysis on Quality Checking of Blood Film Malaria Parasite, National Malaria Elimination Programme, Ministry of Health Malaysia},
author = {Zulhainan H and Kumuthamalar S and Norizat Y and Maimunah A and Meliskha V and Thevarajah DP and Siti Noor Aishah AH and Norazimah S and Mohd Hafizi AH and Jenarun J and Nor Zahrin H},
url = {https://apcph.cphm.my/wp-content/uploads/2022/07/APCPH2022-P-8.pdf
https://apcph.cphm.my/wp-content/uploads/wpforms/1176-1e04940bb5d885bf8711ed19095a89ed/3.-ACPCPH-2022-Poster-Presentation-Dr-Zulhainan-Hamzah-4a052414b9eee02f61e3f4252d846c01.pdf},
year = {2022},
date = {2022-08-02},
urldate = {2022-08-02},
issue = {7},
abstract = {Background: The quality checking of blood film for malaria parasite is an important activity for monitoring the performances of malaria microscopy. Considering its important roles in the Malaria Elimination Programme in Malaysia, we evaluated the performance of the quality checking of blood film malaria parasite carried out between 2015 to 2021 and analyze the outcomes. Methodology: A total of 67,138 blood films malaria parasite were received and analysed for quality checking at the National Public Health Laboratory, of which a total of 18,550 and 48,588 were positive and negative slides, respectively. The microscopy examinations were performed by WHO level 1 (expert) certified malaria microscopist. Results: Analysis showed 99.82% or 67,015 slides were in good agreement with the National Public Health Laboratory readings and only 0.19% or 126 slides were considered as false reading or errors. Of the 126 false reading or errors, 105 were from the positive slides while another 21 were from the negative slides. The percentages of false positive and false negative slides were at 0.17% and 0.04%, respectively. Conclusion: There are still inaccuracies in the diagnosis of all species of malaria parasites, especially Plasmodium knowlesi. The present assessment also revealed that there is a needs for continuous refresher training on malaria microscopy, monitoring of the quality of blood smears preparation and to strictly adhere to the standard procedures of using blood in EDTA tube for preparing the blood film for malaria parasite. In general, the performance of quality checking for blood film malaria parasite are generally satisfactory with the percentage of errors lower than 1%, below the standard set by the National Malaria Elimination Programme, Ministry of Health Malaysia.},
howpublished = {published online at https://apcph.cphm.my},
note = {Type: POSTER PRESENTATION; Organisation: National Public Health Laboratory, Ministry of Health Malaysia, Vector Borne Disease Sector, Disease Control Division, Ministry of Health Malaysia},
keywords = {Blood films for malaria parasite, Malaria microscopy, National Malaria Elimination Strategic Plan.Enter description here., Quality checking},
pubstate = {published},
tppubtype = {proceedings}
}