Abstract
Technique development plays an important role in the field of electron microscopy. In this study, the Rapid Method for Transmission Electron Microscopy (RMTEM) was compared the Standard Method for Transmission Electron Microscopy (SMTEM). By doing this we expect that the RMTEM to be the better choice and would give the electron microscopists a better understanding of the ultra structure of the Staphylococcus aureus ATCC 25923. The bacteria sample preparation showed that RMTEM had significantly higher mean grey values as compared to the SMTEM (t(58) = 6.614, p<0.001; CI95 = 321.329, 600.271). This demonstrates that the contrast produced for RMTEM is lower than the SMTEM. This is due to the inability for bacterial protein and lipid constituents to reorganize into a more compact configuration. Furthermore, the RMTEM would cause less extraction of protein and lipid constituents compared to the SMTEM. This was proven by the thickness of the mean outer membrane of the bacteria that were processed by the RMTEM compared to the SMTEM (t(58) = 8.460, p<0.001; CI95 = 7.2853, 11.8014). However, the mean cross sectional area of the bacteria is significantly reduced in the RMTEM compared to the SMTEM (t(58) = -4.202, p<0.001; CI95 = -40078.459, -14215.007). The later finding indirectly shows that the SMTEM would cause damages to the bacteria 's outer membrane and the plasma membrane causing an increase in bacteria cell size. This could be due to the movement of water molecules from the environment to the intracellular compartment of the bacteria, caused by the protein and lipid extraction from the outer membrane and plasma membrane of the bacteria. With such properties, the RMTEM would be the better choice in providing better detailed images for the ultra structures of the bacteria compare to the SMTEM.
Links
- https://apcph.cphm.my/wp-content/uploads/2022/07/APCPH2022-P-94.pdf
- https://apcph.cphm.my/wp-content/uploads/wpforms/1176-1e04940bb5d885bf8711ed1909[...]
BibTeX (Download)
@proceedings{APCPH2022-P-94,
title = {Rapid Method For Transmission Electron Microscope Study Of Staphylococcus aureus ATCC 25923},
author = {A. S. Santhana Raj and Norzarila Sulaiman Najib and Nur Afrina Muhamad Hendri},
url = {https://apcph.cphm.my/wp-content/uploads/2022/07/APCPH2022-P-94.pdf
https://apcph.cphm.my/wp-content/uploads/wpforms/1176-1e04940bb5d885bf8711ed19095a89ed/Poster-ACPCH-298110f9adac8bd43b0422b0bf7e4bc9.pdf},
year = {2022},
date = {2022-08-02},
urldate = {2022-08-02},
issue = {7},
abstract = {Technique development plays an important role in the field of electron microscopy. In this study, the Rapid Method for Transmission Electron Microscopy (RMTEM) was compared the Standard Method for Transmission Electron Microscopy (SMTEM). By doing this we expect that the RMTEM to be the better choice and would give the electron microscopists a better understanding of the ultra structure of the Staphylococcus aureus ATCC 25923. The bacteria sample preparation showed that RMTEM had significantly higher mean grey values as compared to the SMTEM (t(58) = 6.614, p\<0.001; CI95 = 321.329, 600.271). This demonstrates that the contrast produced for RMTEM is lower than the SMTEM. This is due to the inability for bacterial protein and lipid constituents to reorganize into a more compact configuration. Furthermore, the RMTEM would cause less extraction of protein and lipid constituents compared to the SMTEM. This was proven by the thickness of the mean outer membrane of the bacteria that were processed by the RMTEM compared to the SMTEM (t(58) = 8.460, p\<0.001; CI95 = 7.2853, 11.8014). However, the mean cross sectional area of the bacteria is significantly reduced in the RMTEM compared to the SMTEM (t(58) = -4.202, p\<0.001; CI95 = -40078.459, -14215.007). The later finding indirectly shows that the SMTEM would cause damages to the bacteria 's outer membrane and the plasma membrane causing an increase in bacteria cell size. This could be due to the movement of water molecules from the environment to the intracellular compartment of the bacteria, caused by the protein and lipid extraction from the outer membrane and plasma membrane of the bacteria. With such properties, the RMTEM would be the better choice in providing better detailed images for the ultra structures of the bacteria compare to the SMTEM.},
howpublished = {published online at https://apcph.cphm.my},
note = {Type: POSTER PRESENTATION; Organisation: Electron Microscopy Unit, Institute for Medical Research (IMR), National Institutes of Health (NIH), Selangor},
keywords = {en bloc, fixation, rapid method, Staphylococcus aureus, transmission electron microscope},
pubstate = {published},
tppubtype = {proceedings}
}