Abstract
INTRODUCTION: Environmental exposure damages the DNA. Alarmingly, some environmental toxins are carcinogenic. Thus, this study aimed to associate DNA damage and concentration of heavy metals (HMs) namely Arsenic (As), Cadmium (Cd) and Mercury (Hg) in hair samples of Malay women. METHODS: A cross-sectional study was conducted among Malay women aged 18 to 45 years old. Anthropometric measurements and sociodemographic data were collected. Concentration of HMs was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Meanwhile, degree of DNA damage was detected using Comet assay. Minimum 50 cells were randomly selected and scored using online software (TriTek CometScore 2.0). Levels of the DNA damage was expressed in % Tail DNA, tail moment, tail olive moment and tail intensity. Data analysis was performed using IBM SPSS Statistics 23. RESULTS: Ninety participants with mean age 29.66±5.96 years and no prior occupational exposure to HMs were recruited. Mean concentrations quantified were As (0.15±0.40) ?g/kg, Hg (1.25±4.93) and Cd (7.25±4.67). The basal level of DNA strand breaks was 11.31±5.54 (%DNA in tail mean±SD). Participants of 20-23 years old had significantly higher concentration of Cd (p<0.05). Moreover, higher Cd concentration was significantly associated with higher DNA damage among those 24-27 years old (p<0.05). DISCUSSION: Higher concentration of Cd have been evidently reported among cancer patients in previous studies. Thus, postulating higher incidences of cancer among young age group. Focus attention should be given to identify possible source of HMs contamination among young Malay women.
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@proceedings{APCPH-2019-45,
title = {DNA Damage and Heavy Metals (Cd, As and Hg) in Hair of Malay Women},
author = {Ng Chiat Yin and Mrjan Sadat Seghayat and Normina Ahmad Bustami and Eugenie Tan Sin Sing and Farahnaz Amini},
year = {2019},
date = {2019-07-22},
urldate = {2019-07-22},
journal = {6th Asia-Pacific Conference on Public Health 2019 Proceedings},
issue = {6},
abstract = {INTRODUCTION: Environmental exposure damages the DNA. Alarmingly, some environmental toxins are carcinogenic. Thus, this study aimed to associate DNA damage and concentration of heavy metals (HMs) namely Arsenic (As), Cadmium (Cd) and Mercury (Hg) in hair samples of Malay women. METHODS: A cross-sectional study was conducted among Malay women aged 18 to 45 years old. Anthropometric measurements and sociodemographic data were collected. Concentration of HMs was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Meanwhile, degree of DNA damage was detected using Comet assay. Minimum 50 cells were randomly selected and scored using online software (TriTek CometScore 2.0). Levels of the DNA damage was expressed in % Tail DNA, tail moment, tail olive moment and tail intensity. Data analysis was performed using IBM SPSS Statistics 23. RESULTS: Ninety participants with mean age 29.66±5.96 years and no prior occupational exposure to HMs were recruited. Mean concentrations quantified were As (0.15±0.40) ?g/kg, Hg (1.25±4.93) and Cd (7.25±4.67). The basal level of DNA strand breaks was 11.31±5.54 (%DNA in tail mean±SD). Participants of 20-23 years old had significantly higher concentration of Cd (p\<0.05). Moreover, higher Cd concentration was significantly associated with higher DNA damage among those 24-27 years old (p\<0.05). DISCUSSION: Higher concentration of Cd have been evidently reported among cancer patients in previous studies. Thus, postulating higher incidences of cancer among young age group. Focus attention should be given to identify possible source of HMs contamination among young Malay women.},
note = {Type: ORAL PRESENTATION; Organisation: School of Healthy Aging, Medical Aesthetics, Regenerative Medicine, Faculty of Medicine and Health Sciences, UCSI University, Faculty of Applied Science, UCSI University, Kuala Lumpur, Malaysia},
keywords = {DNA Damage, Heavy Metals; Hair Scalp},
pubstate = {published},
tppubtype = {proceedings}
}